Search results for " oxidative stress inhibition"

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Sildenafil protects epithelial cell through the inhibition of xanthine oxidase and the impairment of ROS production

2009

Recent reports suggest that xanthine oxidase (XO), a modified form of the native xanthine dehydrogenase enzyme, plays an important role in various forms of ischemic and vascular injuries, inflammatory diseases, and chronic heart failure. The XO inhibitors allopurinol and its oxidation product oxypurinol held considerable promises in the treatment of these conditions both in experimental animals and in human clinical trials. More recently, an endothelium-based protective effect of sildenafil, a well-known type-5 phosphodiesterase inhibitor, has been reported in preconditioning prior to ischemia/reperfusion in healthy human subjects. Based on the structural similarities between allopurinol an…

Xanthine OxidasePurinonesEndotheliumCell SurvivalSildenafilIschemiaAllopurinolPharmacologyBiochemistryPiperazinesSildenafil CitrateStructure-Activity Relationshipchemistry.chemical_compoundSettore BIO/10 - BiochimicaTumor Cells CulturedmedicineHumansSulfonesXanthine oxidaseNADPH oxidasebiologybusiness.industryEpithelial CellsGeneral Medicinemedicine.diseasemedicine.anatomical_structurechemistryBiochemistryPurinesCell cultureSettore BIO/14 - Farmacologiabiology.proteinReactive Oxygen SpeciesZaprinastbusinessXanthine oxidase ROS production oxidative stress inhibition sildenafil zaprinast human mammary epithelial cellsmedicine.drugFree Radical Research
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Sildenafil protects human mammary epithelial cells against ROS production induced by estradiol

2010

Several studies suggest that xanthine dehydrogenase (XDH) and its oxidase form (XO) play an important role in various types of ischemic and vascular injuries. Recently, we have demonstrated that estradiol (E2) induces a significant decrease of the expression and activity of XDH and of its conversion to XO in human mammary epithelial cells. E2 is known to induce upregulation of eNOS gene expression in aortic endothelial cells. Because the XO-derived O2·- combines with ·NO to yield ONOO-, and considering that ONOO- converts XDH to XO, the resulting increase of XO activity and reactive oxygen species production would eventually lead to a further increase of ONOO- production, thus creating a vi…

chemistry.chemical_classificationOxidase testmedicine.medical_specialtyReactive oxygen speciesNADPH oxidasebiologyEndocrinology Diabetes and MetabolismPhosphodiesteraseGeneral Medicinemedicine.disease_causeMolecular biologyEndocrinologyEnzymeEndocrinologyDownregulation and upregulationchemistryXanthine dehydrogenaseSettore BIO/10 - BiochimicaInternal medicinemedicinebiology.proteinMolecular BiologyOxidative stressestradiol (E2) human mammaty epithelial cells (HMECs) oxidative stress inhibition reactive oxygen species (ROS) production sildenafil xanthine dehydrogenase (XDH) xanthine oxidase (XO).Hormone Molecular Biology and Clinical Investigation
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